Abstract
All eukaryotic cells including plant cell have the vesicular transport system.
The plant cell has two functionally distinct vacuoles, which are the protein storage vacuole (PSV) and the lytic vacuole. The presence of more than one type of vacuole in plant cells suggests a more complex sorting pathway than that found in other eukaryotes.
In fact, various transport pathways, which are mediated by different kind of vesicles, exist in a single plant cell at the same time. However, molecular mechanisms on the complicated plant membrane traffic are still largely unknown.
The specificity of vesicle fusion is mediated by membrane-associated proteins called SNAREs
To characterize the complex protein transport routes of plant, the sub-cellular localizations v-SNAREs need to be determined.
In this study, we have systematically characterized 24 different v-SNARE molecules, and determined their subcellular localizations by using GFP-fusion proteins.
