Abstract
To explore the protein-protein interactions, seed proteins were investigated by two-dimensional electrophoresis and isotope coded affinity tag labeling followed by ESI Q-TOF mass spectrometry in the wheat line with fine deletion in chromosome 1B. The gene locations of proteins on chromosome 1B were identified by using this line, and quantitative changes of proteins which were related to the chromosome deletion, were precisely determined. Out of the 19 proteins identified by mass fragment analysis of the euploid, ten were down-regulated and four up-regulated, and four did not show any change due to the terminal deletion of chromosome 1B. Selected peptide fragments were subjected to MS/MS analysis for sequence information. The resultant sequence information was submitted to databases for protein identification, and several proteins such as alpha-amylase/subtilisin inhibitor and 20S proteasome alpha-7 subunit were identified. These results show that the lines with fine chromosome deletion can be used to understand protein-protein interactions.
