Abstract
We are studying signaling pathways in rice disease resistance. Our previous studies indicated that OsRac1, a small GTP-binding protein, functions as a regulator of NADPH oxidase in response to pathogens and elicitors. We analyzed proteins, which were expressed differently in cultured cells of wild type, constitutive active and dominant negative OsRac1 transgenic rice and the lesion-mimic mutants (cdr) by 2-D gel electrophoresis and mass spectrometry by using LC/ESI Q-Tof. Interesting spots were excised from 2-D gels and subjected to trypsin digestion. These peptide fragments were identified by mass spectrometry. We are also analyzing differentially expressed proteins isolated from elicitor-treated rice cells. In another study, we isolated OsRac1-interacting proteins by affinity chromatography and analyzed by mass spectrometry. Results of these studies will be presented.
