Phenome analysis of Arabidopsis transposon-tagging lines I

Abstract

Arabidopsis is a model plant in which it is possible to carry out saturation mutagenesis for every coding gene. We constructed about 12,000 Ds transposon-mutated lines as a useful resource in Arabidopsis functional genomics, and sequenced the flanking region of Ds insertion for all independent lines. In our resource, we can investigate about 4,000 genes which would be disrupted by Ds insertion in coding regions. Recently, we have started a "phenome analysis", in which we observe various phenotypes systematically for each Ds insertional line. We are collecting phenotypic data for morphological observation on plant growth for 3,000 insertional lines so far. We classified the results into one hundred kinds of category of phenotypes.