Abstract
An endogenous retrotransposon of rice, Tos17, is inactive under normal conditions. Tos17 is activated by tissue culture and inactivated again in regenerated plants. These features of Tos17 make it suitable for the functional analysis of rice genes by gene disruption. More than 50,000 disruption lines have been produced. From 6,000 lines, 47,000 flanking sequences of Tos17 are determined.
Phenotype data from all disruption lines will be obtained in the next year. We constructed a relational database containing nucleotide sequences of flanking region of Tos17 insertions and phenotype data observed in the rice field. We will introduce in silico mutant screening using our web-based relational database (http://tos.nias.affrc.go.jp/) and some points for analyzing the mutant lines.
