Abstract
About 10,000 non-redundant Arabidopsis full length cDNA (fl-cDNA) clones were mixed to make a normalized fl-cDNA over-expression T-DNA library. Agrobacterium was transformed with the normalized T-DNA library to give the corresponding Agrobacterium library. A. thaliana WT plants were dipped with the Agrobacteria library. About 1200 transformed plants were grown in green house to isolate 93 plants, which showed obvious phenotypes such as morphological abnormality, greening and pigmentation. PCR was performed to amplify 106 fl-cDNA fragments from these lines using vector specific primers. Average size of the fragments was 1,4 kb with the range between 0,3 kb and 4,2 kb. The PCR fragments were sequenced to find out that all such 43 fl-cDNA inserted newly in the genome was not redundant. We designated this activation tagging-like system as Fox Hunting System (fl-cDNA over-expressor gene hunting system) to develop it as a novel technology for the functional genomics of heterologous genomic materials.
