Arabidopsis encyclopedia using full-length cDNAs and its application for functional genomics

Abstract

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. Using the biotinylated CAP trapper method, we have constructed full-length cDNA libraries from Arabidopsis plants and isolated 224,641 RIKEN Arabidopsis full-length (RAFL) cDNA clones. They were clustered into 18,127 nonredundant cDNA groups, about 70% of predicted genes¹⁾. We have determined full-length cDNA sequences of 13,181 RAFL cDNA clones²⁾. We have also used the RAFL cDNAs for the microarray analysis^{3), 4)} of expression profiles and the structure analysis of Arabidopsis genes. In this meeting, overview of our RAFL cDNA project will be presented.
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