Abstract
We have recently proposed that LHY and CCA1 are essential components for circadian clock function in Arabidopsis. Gain-of-function mutation of lhy (lhy-1) causes late flowering under long days (LD) and elongated hypocotyl phenotypes. Loss-of-function mutations of lhy (lhy-11, 12 and 13) were isolated as intragenic suppressors and causes early flowering phenotype in short days (SD). We have screened for mutations that suppressed the early flowering phenotype of the lhy-12 mutant under SD. Here we demonstrate isolation of a new allele of lhy (lhy-2) as an intragenic suppressor of the lhy-12. The lhy-2 is quite similar to the lhy-1 in terms of late flowering under LD and long hypocotyl phenotypes. We also show the lhy-12 has a point mutation in the end of the 5th intron and this causes mis-splicing of LHY. The mis-splicing seems to be partially suppressed by the lhy-2. We will report details on characterization of the lhy-2.
