Abstract
We have studied on an Arabidopsis MADS-box gene, AGL24 which function in the promotion of flowering. Interestingly, SVP which represses flowering, is phylogenetically very close to AGL24. Recent works suggest that MADS-box proteins form complexes with other MADS-box proteins. To reveal whether AGL24 protein form complexes with other MADS-box proteins involved in flowering, proteins interacting with AGL24 were screened by yeast two-hybrid method. As a result, several MADS-box genes such as SOC1, AP1 and FUL were isolated. Studies with yeast experiments indicated that SVP also interacted with all of them. The interactions among these proteins were confirmed by in vitro binding assays. Based on the expression patterns of these genes, AGL24 may interact with SOC1 in shoot apical meristems during vegetative development and with AP1 and FUL in early floral meristems.
