Preparation and Biological Activity of Molecular Probes for Jasmonic Acid-Binding Proteins

Abstract

Several types of jasmonic acid (JA) derivatives, including JA-amino acid conjugates, a JA-biotin conjugate, a JA-dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify and analyze JA-binding proteins. These JA derivatives except JA-fluoresceine conjugate exhibited significant biological activities in rice seedling assay, soybean phenylalanine ammonia-lyase-inducing assay, and/or rice phytoalexin-inducing assay. These results indicate that these JA derivatives might be useful probes to identify and analyze JA-binding proteins. It was also indicated that the activity spectra of prepared compounds were different from each other, suggesting that different types of JA-receptors function in the respective bioassays.