Abstract
Expressed sequence tag (EST) library was prepared from a model tobacco BY-2 cell culture. This EST library includes approximately 10,000 partial cDNA clones that are now opened to public users at (http://mrg.psc.riken.go.jp/strc/). We also carried out multiple microarray analyses with the selected EST clones, and with particular interest in the plant hormone-regulated gene expression. As one example, BY-2 cells cultivated under auxin-deficient conditions were treated with stress-related hormones salicylic acid, abscisic acid and methyl jasmonate. The expression patterns induced by these hormones with a special focus in commonly and/or differentially regulated genes, and the correlation of gene expression with the hormone-induced phenotypes, will be discussed.
