Abstract
We have been studying mechanism, dynamics and physiological roles of endocytosis in Arabidopsis by focusing our attention on endocytic Rab/Ypt GTPases. We previously reported that Ara6, a plant unique Rab GTPase with the highest similarity to mammalian Rab5, and Ara7 and Rha1, conventional type Rab5 homologs, are all on the subpopulation of endosomes. Using fluorescent proteins and CSLM, we examined whether these proteins are on the same endosomes or on the different population of endosomes. We also compared the subcellular localization between Rab5 homologs and some putative endosomal SNAREs, which revealed that Ara6 is localized on the later compartment in the endocytic pathway. On the other hand, Ara7 and Rha1 are on the different population of endosomes from Ara6, where GNOM-dependent recycling should occur. We also show some data suggesting transition of these endosomes from one to another.
