Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 45
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Protein aggregates are transported to vacuole by autophagic mechanisms under nutrient starvation
*Kiminori ToyookaMasaki TakeuchiYuji MoriyasuHiroo FukudaKen Matsuoka
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Pages 327

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Abstract
BY-2 cells transformed with a cytochrome B5 and RFP fusion construct (CytB5-RFP). This protein distributed in the log-phase cells as punctate pattern. Upon reaching to the stationary-phase, some of the RFP fluorescence was detectable in the lumen of the vacuole. To address what is the trigger of such the relocation of CytB5-RFP at the stationary-grown cells, we incubated the cells with different medium and found that nutrient starvation caused the relocation of RFP fluorescence into the vacuole. To examine the involvement of autophagy for the relocation, we expressed the fusion protein of YFP and Apg8 homolog of BY-2. The YFP fluorescence was detectable in the cytoplasm with few punctate spots. The punctate spots increased under starvation conditions, and some of them appeared to be colocalized with some CytB5-RFP aggregates. These findings suggest that BY-2 cells upon starvation use the autophagic process for degradation of the aggregates.
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© 2004 by The Japanese Society of Plant Physiologists
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