Abstract
In Arabidopsis, root epidermal cells differentiate either into hair cells or into hairless cells. One of the R3-type Myb gene, CAPRICE (CPC) is a positive regulator of the hair-cell differentiation and CPC is preferentially transcribed in hairless cells. By the analyses of the CPC promoter, we identified the cis-acting region which is responsible for the hairless-cell-specific transcription. This region contains two putative Myb protein binding sites (pMBS). When the second pMBS was mutated, hairless-cell specific transcription was not observed. R2R3-type Myb protein, WEREWOLF (WER) is considered to be a positive regulator of the hairless-cell differentiation. We showed that WER could bind to the pMBS of the CPC promoter by yeast one-hybrid analysis and gel shift assay. We will discuss about the regulatory system of the CPC transcription and the epidermal-cell differentiation.