Abstract
Intercellular communication is a crucial process to build the multicellular organism such as higher plant. Cell-to-cell movement of macromolecule has been thought to play an important role in this communication.
CPC:GFP fusion protein can move from cell-to-cell in epidermal cells in Arabidopsis root. Two motifs in CPC, one in the N-terminal region and the other in the Myb domain, are required for cell-to-cell movement. Amino acid substitution experiment indicated that both W76 and M78 are critical for cell-to-cell movement of CPC.
To elucidate the molecular machinery for cell-to-cell movement of CPC protein, we isolated the CPC-interacting proteins by yeast two-hybrid screening. One clone encodes the novel protein which has a D/E/K rich domain. This clone did not interact with bait containing W76A mutation, suggesting that this protein was involved in cell-to-cell movement of CPC.
