Abstract
Plant growth is limited in acid soil because of phosphorus (P) deficiency and aluminum (Al) toxicity. Though specific gene expression of rice (Oryza sativa L.) under aluminum and phosphate stress was well investigated, response via protein expression needs further analysis.
To evaluate the differential expression of protein, 1) rice in the seventh leaf stage were subjected to +P treatment, -P treatment, or +Al treatment. Samples were taken at 0,12,24,72,120 hours after each treatment started and at 10 days after treatment in the case of ±P treatment.2) Root proteins were extracted using Tris-HCl/NaCl buffer, then concentrated. The extract was resolved in 7M Urea/2M Thiourea buffer. 3) These proteins were separated by using two-dimensional electrophoresis, then quantified by digital analysis and analyzed by MALDI-TOF-MS or Edman sequencing. As a result, 15 proteins were identified (November, 2003). Based on these data, we examine metabolic changes in low phosphorus or high aluminum treatment.
