Abstract
We have developed a practical method for the comprehensive analysis of sugar phosphates by high performance anion exchange chromatography with pulsed amperometric detection coupled with a titanium oxide column as a trap-column. Using this system, levels of inorganic phosphate, sugar phosphates and a nucleotide phosphate from Arabidopsis grown at three different Pi concentrations in nutrient media, were investigated. These compounds were also measured in the Pi-related mutants (pho1 and pho2). Glc 1-P, Glc 6-P, Frc 6-P, Gal 1-P and Man 1-P increased in proportion to increases in the in vivo level of Pi in wild type plants. In contrast levels of Suc 6-P and UDPG decreased as the in vivo Pi levels increased. Except for Suc 6-P and UDPG, the responses to Pi level in the mutants were similar in wild type plants. However Suc 6-P and UDPG responded differently.
