Elucidation Of Anthocyanin Accumulation Mechanism By "Omics" Approach Using PAP1 Over-expressing Mutants (2)
-Functional Analysis Of Candidate Genes-

Abstract

The pap1-D, activation tagging mutant over-expressing Myb-like transcription factor PAP1, specifically over-accumulates anthocyanins. Genes upregulated in pap1-D were expected to be involved in biosynthesis and sequestration of anthocyanins. Microarray analysis revealed that genes upregulated in pap1-D included those encoding enzymes involved in anthocyanin biosynthetic pathway, transcription factors such as Myb and WRKY, and glycosyltransferases (GTs), acyltransferases (ATs) and glutathione-S-transferases (GSTs) whose function were remained undetermined. Those GTs, ATs and GSTs were expected to be involved in modification of aglycons and sequestration of anthocyanin.
Out of those candidate genes, genes encoding GTs were cloned, and recombinant proteins were expressed in E. coli. Enzymatical properties of those recombinant GTs were analyzed. Metabolomic analysis of A. thaliana mutants that defective in each gene was also performed. Roles that each gene performed in planta will be discussed.