Abstract
This paper presents novel methods of manipulation of single chromosomal DNA molecules for single molecular genomic studies. Chromosomal DNA molecules are, first, extended to the full length by electroosmotic flow. We demonstrated translocation and winding of the extended DNA fibers in solution using optically-driven microstructures. An extended DNA fiber is translocated with a micro-fabricated hook driven by a focused-laser beam. When two optically-driven micro-fabricated bobbins are made into contact with a DNA fiber and the bobbin revolved around the other, the fiber is wound and suspended between them. The combination of the partial DNA manipulation using the hook and the whole manipulation using the bobbins leads to the chromosomal DNA-protein interaction assay in single molecular level.
