Abstract
Both the private and public sectors have be active in researches of the metabolic related genes in the various plants which produce industrially useful materials. A functional genomic approach to primary and secondary metabolism in a model legume plant, Lotus japonicus has led to give significant information on the metabolic pathways common in legume plants. In this study, we investigated a variety of potential effectors, salicylic acid, methyl jasmonate, abscisic acid, gibberellic acid and yeast extract, for their ability to induce transcripts of metabolic related genes in L. japonicus suspension-cultured cells. cDNA macroarray analysis using total RNA isolated from yeast extract-treated and non-treated cells showed the induction of several genes associated with phenylpropanoid and phytoalexin pathways. Currently we are working in the effects of Nod factor and reduced glutathione on expression of L. japonicus specific genes using the suspension-cultured cells.
