Abstract
Analyses of complete genome sequences of seed plants, Arabidopsis and rice were performed. In contrast, there is little knowledge about fern genomes. To understand the genetic information of a fern, Adiantum capillus-veneris, normalized cDNA library was constructed from prothallia grown under white light and analysis of expressed sequence tag (EST) was carried out. Approximately 10,000 clones were sequenced and clustering of these obtained sequences was performed. As a result, 7,132 non-redundant groups were generated. These groups were subjected to similarity search for identification of the putative function. Approximately 1,600 EST groups were found to be similar to sequences of genes registered in the public database. About 1,100 EST groups showed similarity to sequences of unknown function. The remaining EST groups showed no significant similarity and were classified as novel sequences.
