Phenome Analysis of Arabidopsis transposon-tagging Lines II

Abstract

The functions of two-thirds of Arabidopsis genes are remained to be unknown. One way to understand the function of each gene in Arabidopsis is to observe the phenotype of loss-of-function mutant of each gene. To do this analysis, we used Ds transposon-tagging line (ecotype Nossen), in which the flanking region of Ds insertion site was sequenced.
We have already observed the aerial portions of 3,000 Ds-insertion lines and have identified about 100 lines with abnormal phenotype of the aerial portion in F3 generation. We are also checking the phenotype of the seedling in F4 generation. Growing seedlings on agar media, we observed the phenotypes of primary roots, lateral roots, the root-hairs, hypocotyls, and trichomes, and identified a short-root mutant and a short-hypocotyl mutant. In the near future, we will make the data available for Arabidopsis research society.