Abstract
There are at least five types of peroxisomes in higher plants (glyoxysomes, leaf peroxisomes, cotyledonary peroxisomes, root peroxisomes and unspecicalized peroxisomes). During the postgerminative growth of seedlings, a functional transition from glyoxysomes to leaf peroxisomes occurs. Glyoxysomes contain enzymes for fatty acids β-oxidatin and the glyoxylate cycle that implicate to lipid metabolism. Leaf peroxisomes contain glycorate pathway in photorespiration. We identified recently from glyoxysomal proteomic analysis of etiolated Arabidopsis cotyledons, the first protein kinase localized on peroxisomal membranes. Protein phosphorylation is involved in the regulation of many other pathways in plants. To investigate regulation in the metabolic differentiation and the functional transition of peroxisomes, we identified systemically phosphorylated proteins from glyoxysomes, peroxisomes at the functional transition stage and leaf peroxisomes, respectively.
