Abstract
We developed an efficient callus induction system from seed of warm season turfgrass, centipedegrass (Elemochloa ophiuroides Hack.) and attempted regeneration from the induced callus. In order to derive the callus from sterilized seed, phytohormone condition was determined. The callus was induced on Murashige and Skoog (MS) agar medium containing 1.0 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) at 28 oC for 2 weeks. In the case of multiplication, it was the best that the callus cultivated on the MS agar plate containing 1.0 mg/l of 2,4-D and 1.0 mg/l of kinetin. Then, when the increased callus was transferred onto the MS agar medium containing only 1.0 mg/l of kinetin, regeneration of shoot was observed by an induction for 3 weeks at 28 oC. On the other hand, a root formation was also observed after one week by retransplanting shoots onto a hormone-free MS agar plate.
