Abstract
It is well known that most Cd in plant tissues binds to some substances (Rauser, 1999), but such Cd-binding substances (CdBSs) in phloem saps have not yet been examined.
In this study, phloem saps were collected from rice plants grown in artificial soil that contained Cd. Phloem saps were fractionated by size-exclusion HPLC, and Cd contents of HPLC fractions were measured by atomic absorption spectrometry. It is found that almost all of Cd in the phloem saps was bound to some organic substances and that the molecular weights of the CdBSs were less than 10 kDa. To acquire more information about the CdBSs, the HPLC fractions that contained Cd were analyzed by mass spectrometry.
Rauser W.E. (1999) Cell Biochem. Biophys, 31; 19-48
