Abstract
Freezing tolerance can be induced in bromegrass (Bromus inermis Leyss) suspension cultures by exposure to low temperature or by exogenous application of abscisic acid (ABA). To understand the mechanisms of freezing tolerance in bromegrass, we analyzed the genes responsive to low temperature and ABA using rice cDNA microarray. We cloned 10 genes induced by cold and ABA and analyzed these expressions.
To determine the function of cloned genes, the gene products expressed in E. coli were purified. And we constructed transgenic plants in an attempt to identify contribution of these genes to freezing tolerance.
