Abstract
In northern Japan, cool temperature in summer often causes a serious decrease in the yield of rice plants mainly caused by unsuccessful fertilization due to damaged anthers. Anthers of rice plants are highly sensitive to coolness at the early microspore stage and damages at this stage result in the decreases of the number of pollen grains and the pollen germination ratio. These decreases are much more noticeable under extra supply of nitrogen for good yield. Differences of protein between cooled and not cooled anthers from plants grown under two nitrogen conditions were analyzed using two-dimensional gel electrophoresis. beta-expansin was decreased under high nitrogen conditions and glyceraldehyde 3-phosphate dehydrogenase was increased by cooling under high nitrogen conditions. This research was funded by Rice Genome Project (Proteome) from the Ministry of Agriculture Forestry and Fishery Japan.
