Abstract
GLP-1 is a peptide hormone that is composed of 30 amino acids. The GLP-1 is a candidate for future therapeutic agent of the diabetes, because it has a function to induce insulin secretion dependent upon glucose level. In order to accumulate modified GLP-1 (mGLP-1) peptide in the endosperm tissue of rice cells, we generated transgenic rice plants, which have contained synthetic gene coding for the mGLP-1 peptide was directly expressed under the control of rice storage protein glutelin GluB-1 promoter in transgenic rice. However, the peptides were not detected in the rice seed due to gene silencing. Then new constructs in which the mGLP-1 was fused to the C-terminus of GFP or tandemly arranged, were introduced into rice plants in order to avoid gene silencing. The transcripts and mGLP-1 peptide were detected in rice seed for these constructs.
