Abstract
Previously, we reported binary vectors applicable for Gateway Cloning Technology. They were made for construction of reporter/tag fusion genes easilly. In this paper, we report the Dual Site Gateway Binary Vector System for cloning of two genes. With this system, reporter / tag sequence is independently fused to two genes. This system is very useful for plant research, because the biochemical analysis of interaction between two genes interested is very important.
In this time, we made the vector containing new recombination sequences attR3- attR4 in addition to attR1-attR2. Using this vector, two genes were able to be cloned in one vector by one step reaction. We also report application of this system.
