Abstract
The Arabidopsis Genome Initiative determined Arabidopsis genomic sequence with 99.99% accuracy at the end of 2000. This achievement is a milestone in plant science, and has opened a new post-genome era for global analysis of gene function and expression. To analyze the function of each Arabidopsis gene, reverse genetics with insertional mutagenesis by transposon and T-DNA tagging lines becomes quite powerful for systematic functional analysis of Arabidopsis genes. Full-length cDNAs are quite important resource not only for the correct annotation of expressed genes but also for further functional analyses of their gene products. We have collected more than 18,000 Arabidopsis full-length cDNAs (70% total genes). Full-length cDNAs were used for the microarray to analyze changes in gene expression profiles in response to environmental stimuli and hormone. We have deposited a part of the cDNAs and tagged mutants in RIKEN BRC for progress of plant science.
