Abstract
Vascular cells differentiate from the procambium. In order to clarify the mechanism of vascular patterning, it is necessary to understand the early stages of procambial development and provascular development just prior to procambium. To identify genes expressed at the early stages of provascular/procambial development, we have created Arabidopsis enhancer-trap lines and screened for the lines expressing a reporter GFP in the provascular/procambium cells. So far, we obtained 367 lines in which GFP fluorescence was detected in the provascular/procambium cells and/or vasculature. We identified the T-DNA insertion sites in 36 lines. Genes near the insertion sites encode, for example, transcription factors, proteases and AUX/IAA proteins. Concerning the lines in which GFP was expressed in vascular initial and provascular/procambium cells, we are currently performing linkage analysis between GFP expression and their T-DNAs. We report GFP expression patterns of the aforementioned lines, their T-DNA insertion sites, and results of the linkage analysis.
