Abstract
In order to identify genes involved in early step of vascular formation, we screened 51000 gene trap lines with GUS as a reporter gene.We isolated two groups of candidate lines, group 1 (show GUS staining pattern preferentially along procambial cells in developmental stage 19 embryo; 3 lines), and group 2 (show abnormal vascular development; 3 lines). We reported previously identification of the tag insertion loci for group 1 lines (GGH, RING finger, unknown gene). Here, we report identification of causal genes of group 2 lines as CYP86, AtKTN, and an unknown gene. We also analyzed detailed expression patterns of these lines. Group 1 genes as well as AtKTN show GUS staining pattern preferentially along procambial cells or meristematic tissues of mature plants. However, each line shows differential expression pattern in root apex region, which may represent different stages in procambial development.
