Abstract
The expression of chalcone synthase (CHS) is stimuated by developemental signals and a variety of environmental stimuli, and the resultant flavonoids have wide range of physiological functions. In particular, leguminous plants are known to synthesize antimicrobial isoflavone, phytoalexin in response to pathogen attack. CHS1, a member of soybean CHS gene family is found to be transcribed after both UV light irradiation and elicitor treatment. Functional analysis of 5'deletions have delimited the sequences nessesary for elicitor inducibility.
We have isolated SBZ1 which binds to promoter region of french bean CHS15 and soybean CHS7 containing both the G-box and the H-box, and becomes a substrate for phosphorylation in vitro. Here we show that recombinant SBZ1 could bind to CHS1 promoter region. Furthermore, this binding to CHS1 promoter was stronger than to CHS7, which indicates that SBZ1 might be involved in elicitor inducibility of CHS1.
