Abstract
Phenylalanine ammonia-lyase (PAL) acts as the key enzyme in regulating metabolic flow from the primary metabolism to phenylpropanoid metabolism, and the genes encoding PAL are regulated by both environmental and developmental cues. It has been revealed that the expression of gDcPAL1 gene in suspension-cultured carrot cells is induced by dilution effect, elicitation and UV-B irradiation, and box-L1 and box-L5 in the gDcPAL1 promoter play an important role to regulation of gDcPAL1 expression. We isolated DcMYB1 transcription regulatory factor, which binds to both box-L1 and box-L5 and have studied on the function of DcMYB1. In this study, it was indicated that gDcPAL1 promoter was activated by DcMYB1 binding to the plural box-Ls in gDcPAL1 promoter and that DcMYB1 acted as the transcriptional activator concerning to the induction of expression of gDcPAL1 by several stresses, they were dilution effect, elicitation and UV-B irradiation.
