Abstract
Deduced functions of AtBCB and NtGDI1 gene for Al stress were investigated. The cDNAs fused to a GFP gene in plant cells showed that the AtBCB and NtGDI1 protein exist in cell membrane and cytoplasmic region, respectively.
Non-transformant (Ler) accumulated lignin by Al stress, while the AtBCB transformant showed a constitutive produce of lignin in root. Microscopic observation of Al treated roots indicated that a deposition of lipid-peroxides was clearly low in the area where lignin was accumulated. We supposed that lipid peroxidation was probably eliminated by lignin.
Temperature sensitive yeast sec19- mutation was complemented by the NtGDI1 gene at 37 degerees. Sec19- mutant showed Al sensitive at 32 degrees and this phenotype was also complemented by NtGDI1 gene. Since Sec19 protein functions for a vesicle transport, we suppose that NtGDI1 protein can function for Al efflux in both yeast and Arabidopsis.
