A strategy for finding silent phenotypes in Arabidopsis using FOX hunting system

Abstract

For investigating the global metabolic changes in ilent phenotypes we introduced metabolomics concept using OX (Full-length cDNA Over-expressor gene) hunting system of model plants, Arabidopsis thaliana. Using the system we are trying to make a rapid and useful method to find the silent phenotypes by GC-TOF/MS.
Five seeds from every FOX hunting lines were grown on the MS agar medium for screening. The aerial part of each 25 days-old FOX line was harvested. Samples were extracted and analysed by GC-TOF/MS, and thereafter the non-processed GC-MS files were subjected to multivariate analysis. The PLS-DA results indicated that several lines had metabolic differences compared with those of other lines. The compounds which made differences contained both primary and secondary metabolites. In the poster, we will present the method of sample cultivation, extraction and the result of multivariate analysis using chemometrics.