Abstract
To study function of nuclear genes involved in chloroplast development and photosynthesis, we have started to collect Ds-tagged knock-out lines that 2090 plastid proteins with a cTP predicted to be encoded by nuclear genomes in Arabidopsis thaliana.
To understand regulatory-networks of metabolite, we obtained metabolic profiles of apg1 (the 37 kDa inner envelope membrane polypeptide related plastoquinone biosynthesis), apg2 (TatC homologue of a ΔpH-dependent protein transporter in thylakoid membrane) and apg3 (translation system in chloroplast) using integration of metabolomics.
FT-ICRMS (Fourier Transform-Ion cyclotron Resonance-Mass spectrometry) is a nontargeted, high-throughput analytical system for metabolomics analysis in which crude by means of direct injection without prior separation metabolites by chromatography. We observed about 500 m/z values (mass peaks) from each analysis of 100% methanol extracts in 3-week-old apg mutants and the results were subjected to PCA. We will discuss roles of metabolites with significant profiles.
