Abstract
NMR might play a comprehensive methodology in the metabolomics studies due to its excellent reproductively, and availability of in vivo measurement. Therefore, we are currently focusing to develop novel methodologies for an NMR-based metabolomics. Especially, we are trying to apply the uniform stable isotope labeling of higher plants for the hetero-nuclear NMR experiments previously used in protein NMR. Use of stable isotope labeling methods has enormous advantage for discrimination of incorporated or de novo synthesized compounds. First, the NMR-based metabolomics revealed that the higher plants can uptake not only inorganic nitrogen sources, but also nitrogen-containing organic compounds. Since the plant uptake of organic compounds is also extremely important issues in applied sciences, we are currently investigating two types of plant culture; Oryza sativa grown in hydroponics and Arabidopsis thaliana grown with soil. Furthermore, potentiality of in vivo NMR metabolomics will be discussed in the conference.
