Abstract
Somatic embryogenesis is unique characteristics of plant cells. Embryogenic cells (EC) of carrot maintained in the medium containing 2,4-D regenerate whole plants via somatic embryogenesis after the depletion of 2,4-D. C-ESE1 (Carrot Early Somatic Embryogenesis 1)was isolated from early stage of somatic embryogenesis after 2,4-D depletion. Transgenic carrot EC with cosuppressed C-ESE1 expression showed that C-ESE1 might be involved in cell attachment in somatic embryogenesis and also affect the polysaccharide deposition on the cell wall.
Here we report the polysaccharides in the medium of C-ESE1-cosuppressed cell culture. The analysis of sugar composition of the polysaccharides isolated from the culture medium indicated that the polysaccharides isolated from C-ESE1-cosuppressed EC culture medium had considerably increased the fucose, arabinose and xylose, suggesting thatC-ESE1 cosuppression induced the loss of some polysaccharides and/or glycoproteins from the cell wall for cell-to-cell attachment.
