Abstract
We reported the purification and partial structure of the glycan elicitor from Alternaria alternata 102 at the last annual meeting. As the further structural analysis, the detail structure was analyzed by reducing sugar analysis, MALDI-TOF/MS and NMR spectroscopy. These results suggested that the elicitor was non-cyclic β-1,3-1,6-oligoglucan and the polymerization of the elicitor was in the range from 8 to 17.
Glucan elicitor could induce class IV chitinases, but not SA and MeJA treatments in BY-2 cells. To better understand an elicitor response signaling, we analyzed glucan elicitor responsible genes using a cDNA microarray, which contained about 16,000 EST. About 300 genes were induced 1h after the treatment with laminarin (β-1,3-glucan) or sugar fraction form A. alternata culture medium. In comparison to data sets of 2,4-D, ABA, BA, MeJA and SA treatments, 14 putative transcription factor genes were identified as an early and glucan elicitor specific inducible genes.
