Abstract
Interaction of suspension-cultured rice cells with rice blast fungus, Magnaporthe grisea, was investigated. Suspension-cultured rice cells were incubated with spores of compatible or incompatible race of M. grisea in the same medium and the production of H2O2, expression of defense-related genes and induction of cell death were analyzed. So far tested, we could not see any significant differences in gene expression profile and induction of cell death. H2O2 was transiently produced and the peak was 2-3 h after inoculation with spores of compatible and incompatible races of M. grisea. Incompatible races induced higher level of H2O2 than compatible races, whereas the addition of spores washed by centrifugation induced the similar level of H2O2 irrespective of compatibility. The supernatant fraction of spore-suspension had H2O2-degrading activity, and the activity disappeared by heating at 100C for 5min, indicating that proteinaceous factors are responsible for the degrading action.
