Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 46
Conference information

Proteome Analysis of OsRac1 Signaling in Defense Response of Rice
*Masayuki FujiwaraKenji UmemuraTsutomu KawasakiKo Shimamoto
Author information
CONFERENCE PROCEEDINGS FREE ACCESS

Pages 400

Details
Abstract
Our previous studies indicate that OsRac1, a small GTP-binding protein, functions as a key regulator of defense signaling in rice . The aim of this study is to reveal the defense signaling pathway in rice by applying the proteome analysis. Proteins extracted from rice cultured cells of wild-type (WT), constitutive active OsRac1 (CA-Rac) and dominant negative-OsRac1 (DN-Rac) were applied to two-dimensional gel electrophoresis (2-DE). The 2-DE images of WT, CA-Rac and DN-Rac were compared and different spots were identified. These spots were identified by mass spectrometry. We also analyzed the differentially expressed proteins isolated from sphingolipid elicitor-treated cells. We detected the different expression level of protein, in which WT, CA-Rac and DN-Rac and identified these proteins. Heat shock proteins (HSPs), defense-related proteins and oxidative stress associated proteins were identified. Additionally, we found up- or down regulated proteins with treatment of sphingolipid elicitor.
Content from these authors
© 2005 by The Japanese Society of Plant Physiologists
Previous article Next article
feedback
Top