Abstract
Plants protect them from pathogen attack by a series of defense response, including generation of reactive oxygen species (ROS) and cell death. The signaling pathway from recognition of pathogens to induction of defense reaction is largely unknown. We have previously shown that OsRac1 is involved in disease signaling pathway, and regulates cell death, production of ROS and phytoalexin production. We focus on identification of proteins contained in the OsRac1 protein complex. Immunoprecipitation from transgenic suspension cultures expressing myc-tagged constitutively active OsRac1, dominant negative OsRac1, constitutively active OsRac1 with membrane localization mutation was analyzed by mass spectrometry. Results showed the OsRac1 complex contained HSP70 that is thought to be involved in defense responses. In addition, identification of other proteins present in the OsRac1 complex will be presented.
