Abstract
To date, >32,000 cDNA clones have been collected and sequenced by the Rice-Full-Length-cDNA Project. Mapping of the cDNAs to rice genome sequence (Pseudomolecule ver 2 from TIGR) indicated that 1094 clones may be transcribed from the different regions of rice genome. Among them, 681 clones may be produced by the trans-splicing event, and we attempted to detect the corresponding transcripts by RT-PCR. A fragment from one of these clones, AK100704, suggested to be generated from two transcripts, which were derived from different chromosomes. They were joined differently from that shown in the previously reported cDNA. The RNA fragment with the same structure was also detected in several tissues such as leaves, stems, roots, and panicles. The corresponding transcriptional origins are located in the chromosome 1 and the chromosome 5, respectively, and no other sequence was found. Therefore, these data suggest that the trans-splicing event may involved creating this transcript.
