Abstract
The Fox Hunting System (Full-length cDNA over-expresser gene-hunting system), a system for random over-expression of a normalized Arabidopsis full-length cDNA library, was applied for the functional analysis of stress-inducible transcription factors. An agro-library containing forty three stress-inducible transcription factors was constructed and used for transformation of Arabidopsis plants. Among 224 T1 transgenic plants, eight transformants expressed DREB1A gene as an internal control, with typical DREB1A-overexpressing phenotypes. Five plants expressing a zinc-finger protein gene showed hyponastic leaves and downward-pointing flowers. In these transgenic plants, expression of several genes involved in SAM development were altered suggesting that the zinc-finger protein has a important role in morphological development as well as in stress responses. In this presentation, we will demonstrate that the Fox Hunting System is useful for the screening of valuable functions from a small pool of genes such as transcription factors showing similar expression pattern.
