Expression profiling using Arabidopsis whole-genome regulatory gene oligo DNA microarray

Abstract

Regulatory genes, such as transcription factors, protein kinases and F-box proteins play critical roles in life cycle of higher plants. Although extensive studies have been carried out for functional analysis of individual regulatory genes, the function of only a small fraction of these regulatory genes has been revealed so far.
It is valuable to learn how the regulatory genes are expressed and regulated at the whole-genome scale. Therefore, we prepared Arabidopsis whole-genome regulatory gene oligo DNA microarray containing all transcription factors, protein kinase and F-box protein genes in Arabidopsis genome recently. The 60-mer oligo DNA corresponding to 1979 transcription factors, 1060 protein kinase and 551 F-box protein genes are spotted on slide glass as a custom array of Agilent Co. In this meeting, we present expression profiles of regulatory genes in various treatments and plant tissues using the Arabidopsis regulatory gene oligo DNA microarray.