Metabolmics using Fourier Transform Ion Cyclotron Mass Spectrometry
1:Development of platform for FT-ICR MS analysis

Abstract

A metabolomics platform on a basis of Fourier Transform-Ion Cyclotron Mass Spectrometry (FT-ICR MS) was implemented for simultaneous analysis of a wide array of metabolites present in crude plant tissue extract. A big advantage to use FT-ICR MS is the applicability for the analysis of crude tissue extracts without any chromatographic separation processes. Another important benefit is the very high resolution, which could lead to the prediction of molecular identity of each metabolite present in such crude extracts. Tools for MS data handling and statistical analysis were also developed to obtain metabolic fingerprinting patterns from FT-ICR MS analyses. In this study, we extracted Arabidopsis cultured cell with 100% methanol, and the crude extracts were subjected to MS analysis using electrospray ionization method. Metabolic fingerprinting patterns were obtained from T87 cells of different culture conditions. Further analysis is underway to identify specific compounds found in cultured cells of P450 gene overexpression.