Abstract
We produced F1 hybrid tomato lines by crossing the miniature tomato cultivar Micro-Tom and the wild tomato Lycopersicon pimpinellifolium and analyzed the metabolites using HPLC and GC-TOF-MS (gas chromatography time-of-flight mass spectrometry). We showed that lycopene content of L. pimpinellifolium in fruit was significantly higher than in Micro-Tom. Lycopene contents of the F1 hybrid fruit were between those of L. pimpinellifolium and Micro-Tom. Moreover, we performed GC-TOF-MS analyses of the metabolites of these tomato lines in biological triplicates. Principal component analysis (PCA) showed that the tomatoes with the same genetic backgrounds were distinguished in their metabolite profiles, suggesting that metabolome profiling might be useful for evaluating genetic traits in breeding.
