Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 46
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A high density multiwell culture system of plant cells in suspension
Ivan Galis*Joji MatsuoHiroo FukudaKen Matsuoka
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CONFERENCE PROCEEDINGS FREE ACCESS

Pages 480

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Abstract
An efficient method to characterize functions of many novel genes, which are discovered by genome analysis, is to overexpress the genes in cells and to analyze the phenotype of transformed cells using suspension culture system. However, because the growth of large numbers of independent plant cells in suspension usually require large facilities, a novel method to culture many independent cell lines with small quantity at the same time should be developed. Therefore, we developed a 96-well culture system using a 96-well deep plate, a gas permeable filter and a newly developed rotator for the 96-well deep plates. By using this system, we could cultivate tobacco BY-2 cells over six weeks without any growth defect under a condition to subculture once a week into flesh medium using a multi-channel pipette. Currently we are testing if this method can also be used to grow rice Oc cells in suspension.
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© 2005 by The Japanese Society of Plant Physiologists
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