Abstract
PAP1 encodes the MYB transcription factor that enhances expression of a set of anthocyanin biosynthesis genes in Arabidopsis thaliana. To elucidate the biosynthesis mechanism, we overexpressed the gene under the strong promoter in the Arabidopsis suspension-cultured T87 cells. Metabolome analyses using high-performance liquid chromatography-mass spectrometry (MS) and gas chromatography time-of-flight MS revealed accumulation of anthocyanins, cyanidin derivatives in the transgenic cells. The transcriptome analysis of the cells using the Agilent 21,500 DNA array chips indicated induction of some anthocyanin biosynthesis genes. In addition, putatively assigned genes for glycosyltransferase and acyltranserase were also induced in the cells, suggesting the involvement of these genes in the anthocyanin biosynthesis. Induction of some genes encoding glutathione S-transferase and transcription factors, which was not shown in transgenic plants, suggests a distinct control mechanism of PAP1 in suspension-cultured cells.
